Cloning vectors and cloning kits - Alpha Universe LLC

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Cloning vectors and cloning kits

Namesize (b.p.)Origin of replicationAntibiotic-resistance markersAdditional featuresApplications
pACYCentry163,397p15Achloramphenicola-complementation, T7 and arabinose promotrersgeneral cloning and expression in E. coli
pACYC-lacZ12,172p15Achloramphenicola-complementationgeneral cloning
pUCentryKm104,751pMB1kanamycin
a-complementation, T7 and arabinose promotrers
general cloning and expression in E. coli
pCampy157,277p15A, Campylobacterchloramphenicoloperon encoding bacterial luciferaseshattling of DNA between E. coli and Campylobacter cells
pAlacZ-SupF105,258pMB1
ampicillina-complementation, supressror tRNA and replication only in the presence of IPTG
engineering of new strains via introduction into targeted cells of different enzymatic activities followed by their fast elimination from modified cells
pi-entry83,190pMB1
nonea-complementation, T7, arabinose and lactose promotrers, supressror tRNA

pACYC-II-Spe10
4,709p15A
spectinomycinproduction of filamentous phage pII replication of plasmids carrying origin of replication of filamentous phage M13
pVLentry-Hyg10




pVLentry-Blast1




pVLentry-Nor1




pVHentry-complete




pVHentry-Hisbio





pUCentryLux Cloning kit6,407pMB1
ampicillin
systems for directional cloning of DNA fragments and positive selection of inserts, T7 promoter and operon encoding protein of interest and bacterial luciferase
fast generation of expression constructs and optimization of expression

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