M13KsupFRNApolT7

  • Model: AUV34
  • 50 Units in Stock

$100.00

Catalog: AUV34.
This Escherichia coli bacteriophage M13 derivative can be used as a trigger for expression of chimera genes controlled by bacteriophage T7 promoter and encoding toxic for the host cell proteins.

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This vector is a derivative of bacteriophage M13 that encodes:
1) RNA polymerase of bacteriophage T7, and
2) supF tRNA that can recognize TAG stop codon as a sense codon.

When packaged in the phage capsid, this vector can be introduced into F+ or Hfr Escherichia coli cells via conventional infection process. The infection does not kill cells nor it causes their lysis. Yet, it will trigger production of full-size proteins whose expression is controlled by T7 bacteriophage promoter and whose coding sequences contain TAG codons introduced to eliminate background expression.

The vector is supplied in 1 ml aliquots of storage buffer containing 40% glycerol and 1013 particles.

This product was added to our catalog on Thursday 14 March, 2019.

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