pCamp-Km10

The plasmid is fully compatible with those carrying the p15A replicon.

This shuttle allows cycling of nucleotide sequences between Escherichia and Campylobacter species.

It is a medium copy number, E.coli plasmid, 5877 b.p. in length.

The plasmid contains:

1) the p15A replicon (ORI) responsible for plasmid replication in Escherichia coli(source - plasmid pACYC184);

2) repB sequence encoding component of Campylobacter episome replication machinery;

3) the hybrid operon that functions in both Escherichia and Campylobacter species and encode aminophosphotransferase conferring resistance to kanamycin (Km(R));

4)  Escherichia coli lactose (Plac) and Campylobacter (Psap) promoters;

5) marked are unique recognition sites for restriction endonucleases.

The plasmid is fully compatible with Escherichia coli plasmids carrying the pMB1 and ColE1 replicons.