pCamp-Lux-Cm15-1

This shuttle allows cycling of nucleotide sequences between Escherichia and Campylobacter species.

The plasmid contains:
1) the p15A replicon (ORI) responsible for plasmid replication in Escherichia coli(source - plasmid pACYC184);
2) sequences encoding components of Campylobacter episome replication machinery (repA and repB);
3) the hybrid operon that functions in both Escherichia and Campylobacter species and encoding both subunits of bacterial luciferase (LuxA and LuxB) and chloramphenicol acetyl transferase Cm(R);
4) Escherichia coli lactose (Plac) and Campylobacter (Psap) promoters;
5) marked are unique recognition sites for restriction endonucleases.

The plasmid is fully compatible with Escherichia coli plasmids carrying the pMB1 and ColE1 replicons.