M13KsupFRNApolT7

This Escherichia coli bacteriophage M13 derivative can be used as a trigger for expression of chimera genes controlled by bacteriophage T7 promoter and encoding toxic for the host cell proteins.

This vector is a derivative of bacteriophage M13 that encodes:
1) RNA polymerase of bacteriophage T7, and
2) supF tRNA that can recognize TAG stop codon as a sense codon.

When packaged in the phage capsid, this vector can be introduced into F⁺ or Hfr Escherichia coli cells via conventional infection process. The infection does not kill cells nor it causes their lysis. Yet, it will trigger production of full-size proteins whose expression is controlled by T7 bacteriophage promoter and whose coding sequences contain TAG codons introduced to eliminate background expression.