pACYC-IRL-lysis30

This vector changes the balance of tRNAs in host cells, thus improving expression of sequences utilizing codons rare used in Escherichia coli cells. Also, it encodes arabinose-induced operon whose expression substantially increase lysis of cells by freeze/thaw cycling.

This vector is a medium copy number, E.coli plasmid, 5017 bp in length.

The plasmid contains:
1) the p15A replicon (ori) responsible for plasmid replication (source - plasmid pACYC184);
2) the Cm(R) gene, coding for chloramphenicol acetyl transferase, which confers resistance to chloramphenicol (source - plasmid pACYC184);
3) genes for E.coli tRNAs recognizing codons: ATA (Ile-tRNA), GAG (Arg-tRNA), CTA (tRNA2), and ATG (tRNA1);
4) the chimera operon encoding components of bacteriophage lambda cell lysis machinery (R and Rz) whose expression is controlled by E.coli arabinose regulatory elements (araC and Para)

The plasmid is fully compatible with those carrying the pMB1 and ColE1 replicons.