pACYC-RNApolT7-lysis1

This vector enables expression of genes controlled by bacteriophage T7 promoter and simplifies lysis of corresponding host Escherichia coli cells.

This vector is a medium copy number, E.coli plasmid, 7610 bp in length.

The plasmid contains:
1) the p15A replicon (ori) responsible for plasmid replication (source - plasmid pACYC184);
2) the Cm(R) gene, coding for chloramphenicol acetyl transferase, which confers resistance to chloramphenicol (source - plasmid pACYC184);
3) the chimera operon encoding RNA polymerase T7 and components of bacteriophage lambda cell lysis machinery (S, R and Rz) whose expression is controlled by E.coli arabinose regulatory elements (araC and Para)

The plasmid is fully compatible with those carrying the pMB1 and ColE1 replicons.